ALLASIA Valérie

The laboratory’s objective is to identify the molecular mechanisms underlying plant susceptibility to pathogenic oomycetes.

The model organism used is the plant Arabidopsis thaliana, which serves as a host for identifying plant functions manipulated by Hyaloperonospora arabidopsidis, an obligate biotrophic oomycete that causes downy mildew on leaves.

UMR Biosafety and Regulated Activities Officer

National Mission: INRAE Scientific Advisor and Evaluator within the Biological Safety Unit

Within the ISC PlantBios:

Referent for the contained facility “TRANSGENESIS”

DEGREES

2012: Diploma from the École Pratique des Hautes Études (EPHE), Paris

1992: Higher National Diploma (BTS) in Biotechnology

AREAS OF EXPERTISE / SCIENTIFIC SPECIALTIES

Plant biology, plant pathology, molecular biology, cell biology, analytical biochemistry, biosafety, regulated activities.

TECHNIQUES / AREAS OF EXPERTISE

In vitro culture, Gateway cloning, RT-PCR, Arabidopsis and Nicotiana transformation, heterologous protein production in E. coli, yeast two-hybrid assays, Western blotting, confocal microscopy, metabolomics…

PUBLICATIONS OVER THE LAST 10 YEARS:

Riglet L, Hok S, Kebdani-Minet N, Le Berre J, Gourgues M, Rozier F, Bayle V, Bancel-Vallée L, Allasia V, Keller H, Da Rocha M, Attard A, Fobis-Loisy I. Invasion of the stigma by oomycete pathogenic hyphae or pollen tubes: striking similarities and differences. J Exp Bot. 2024 Oct 30;75(20):6258-6274. doi: 10.1093/jxb/erae308.

Galli M, Jacob S, Zheng Y, Ghezellou P, Gand M, Albuquerque W, Imani J, Allasia V, Coustau C, Spengler B, Keller H, Thines E, Kogel K-H (2023). MIF-like domain containing protein orchestrates cellular differentiation and virulence in the fungal pathogen Magnaporthe oryzae. iScience 26, 107565. https://doi.org/10.1016/j.isci.2023.107565

Giordano L, Schimmerling M, Panabières F, Allasia V, Keller H (2022). The exodomain of the impaired oomycete susceptibility 1 receptor mediates both endoplasmic reticulum stress responses and abscisic acid signalling during downy mildew infection of Arabidopsis. Mol Plant Pathol, 23, 1783-1791.https://doi.org/10.1111/mpp.13265

Giordano L, Allasia V, Cremades A, Hok S, Panabières F, Bailly-Maître B, Keller H (2022). A plant receptor domain with functional analogies to animal malectin disables ER stress responses upon infection. iScience 25, 103877. https://doi.org/10.1016/j.isci.2022.103877

Testi S, Kuhn M-L, Allasia V, Auroy, P, Kong F, Peltier G, Pagnotta S, Cazareth J, Keller H, Panabières F (2019). An oomycete effector impairs autophagy in evolutionary distant organisms and favors host infection. bioRxiv https://doi.org/10.1101/697136

Allasia V, Industri B, Ponchet M, Quentin M, Favery B, Keller H (2018). Quantification of salicylic acid (SA) and SA-glucosides in Arabidopsis thaliana. Bio-protocol 8(10): e2844. DOI: 10.21769/BioProtoc.2844.

Quentin M, Baurès I, Hoefle C, Caillaud MC, Allasia V, Panabières F, Abad P, Hückelhoven R, Keller H, Favery B (2016).  The Arabidopsis microtubule-associated protein MAP65-3 supports infection by filamentous biotrophic pathogens. https://doi.org/10.1093/jxb/erv564